FastQCFastQC Report
Thu 2 Jun 2016
DRR028355_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028355_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105696
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27300.12964834430041183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24400.11587617585824354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25900.071.961131
GTATCAA49250.061.403681
TCAACGC53600.055.5702634
ATCAACG54850.053.907473
CAACGCA55750.052.923565
AACGCAG57650.051.305086
TATCAAC63200.046.7852022
ACGCAGA65700.044.90857
CGCAGAG67050.043.7880638
GCAGAGT82600.035.544679
GTACATG44450.031.651521
TACATGG49250.028.252732
ACATGGG50700.026.3011843
CATGGGG44600.023.2362794
CAGAGTA80000.021.9293069
AATACCG2959.577783E-619.6592755
ATGGGGG28800.019.1302495
GTACTAG6500.018.9670681
GTATACG2000.00790561118.1302851
AGAGTAC74100.017.81117210-14
Produced by FastQC (version 0.11.5)