FastQCFastQC Report
Thu 2 Jun 2016
DRR028354_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028354_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522910
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45580.2992954278322422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19000.057.5860984
ATCAACG19350.056.540763
GTATCAA20500.055.511541
CAACGCA21650.050.872145
AACGCAG21550.050.0994876
GTACATG48100.045.81061
TATCAAC25150.044.952362
TACATGG47350.041.4777532
ACGCAGA26300.039.9516947
GGTATCA14600.039.2203671
CGCAGAG28600.035.4731068
CATGGGG46400.033.262584
ACATGGG60000.031.1554573
GCAGAGT32650.028.631489
GAGTACA31500.027.3825511
ATGGGGG34600.025.9680825
GTATAAG6800.024.5163821
AGTACAT34700.023.8090062
TAGATCG1902.2022474E-422.8818935
ATAGTAC6750.022.5414163
Produced by FastQC (version 0.11.5)