FastQCFastQC Report
Thu 2 Jun 2016
DRR028354_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028354_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522910
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29360.19278880564183046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27830.18274225003447347No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24400.077.8710251
GTATCAA43900.067.7304151
TCAACGC53750.055.014244
ATCAACG54350.054.4069063
CAACGCA56850.051.653935
AACGCAG57650.051.062916
TATCAAC58550.050.2565352
ACGCAGA66200.043.9202967
CGCAGAG69400.041.9996268
GCAGAGT78950.036.6437239
GTACATG46800.035.6407171
TACATGG46700.034.1428072
ACATGGG47050.033.7347833
CATGGGG44900.030.184834
GTGGTAT11300.027.5965271
ATGGGGG32950.024.6457145
CAGAGTA74800.024.233569
GTAATAC7200.022.1454033
GAGTACA33000.021.9760931
TGGTATC13750.021.0838872
Produced by FastQC (version 0.11.5)