FastQCFastQC Report
Thu 2 Jun 2016
DRR028353_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028353_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974966
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58240.29489115255655035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22450.050.024344
ATCAACG23700.047.0826033
CAACGCA25450.044.6992235
AACGCAG26600.043.5861556
GTACATG57500.042.3559151
GTATCAA28150.041.7137531
GGTATCA18700.041.474851
TACATGG58350.039.6190572
TATCAAC30350.037.727042
ACGCAGA31600.035.7760057
ACATGGG62600.035.7661553
CGCAGAG31900.033.849328
CATGGGG55000.029.6405244
GAGTACA40250.026.6525331
AGTACAT46200.022.2741282
GCAGAGT43550.021.9671279
TCGTGCG1700.00310007221.316149
ATGGGGG45450.018.8120545
TATACTG17150.017.7448885
AGTGCGA2500.001397999217.3926438
Produced by FastQC (version 0.11.5)