FastQCFastQC Report
Thu 2 Jun 2016
DRR028353_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028353_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974966
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28400.14379994389776837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25530.12926804815880374No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25650.072.1081
GTATCAA48450.062.277361
ATCAACG55600.053.8350263
TCAACGC56000.053.191654
CAACGCA58550.050.64545
AACGCAG59650.049.8336
TATCAAC62250.047.9687652
ACGCAGA69200.042.5366747
CGCAGAG71050.041.4291088
GTACATG59100.036.4501761
GCAGAGT84550.034.814179
TACATGG60500.033.6629032
ACATGGG61600.032.2373623
CATGGGG53200.026.4289424
TACGACC2451.9193485E-623.6653864
CAGAGTA80800.022.432229
TACGCCG2205.9056445E-419.7729845
GAGTACA44300.019.1563511
ATGGGGG42250.019.0475855
TTATACT18550.018.75374
Produced by FastQC (version 0.11.5)