FastQCFastQC Report
Thu 2 Jun 2016
DRR028352_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028352_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781055
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51610.28977207329363774No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22150.048.0836454
ATCAACG22850.045.6593863
GTATCAA26350.045.3854061
GGTATCA15000.043.970671
AACGCAG24900.042.775616
CAACGCA25300.041.526545
TATCAAC31050.037.575412
GTACATG44450.037.0140841
ACGCAGA28700.036.612187
CGCAGAG29200.035.7380948
TACATGG45900.031.2602162
ACATGGG48000.027.9244253
GTACGAC1400.001003590125.8759173
CATGGGG42200.024.8948384
GCAGAGT37850.024.3172269
GAGTACA31450.023.5067421
TAGTACG2857.143366E-620.3375634
ACCGCGT1800.00431907420.130258
TAGATCG2659.397924E-519.1394925
GTATAAG9600.018.1197811
Produced by FastQC (version 0.11.5)