FastQCFastQC Report
Thu 2 Jun 2016
DRR028352_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028352_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781055
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38860.21818528905620543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33620.1887645243970568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18130.10179359986075669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26900.079.538491
GTATCAA51450.063.8587571
ATCAACG60400.053.516593
TCAACGC60250.053.4092374
CAACGCA63700.049.861935
AACGCAG64200.049.8124546
TATCAAC66900.048.2112922
CGCAGAG73850.042.8124858
ACGCAGA73700.042.801227
GCAGAGT84350.037.0532729
GTACATG43600.032.1054271
TACATGG43250.029.8296952
ACATGGG44000.028.6607173
AACGCCG1409.984724E-425.8986056
CATGGGG39400.025.200834
GTGGTAT15100.024.4963191
CAGAGTA83100.024.2592959
TTAGCGC1801.5186473E-424.1720318
TAGCGCA1952.6119294E-422.3126459
TGGTATC15600.022.3013692
Produced by FastQC (version 0.11.5)