FastQCFastQC Report
Thu 2 Jun 2016
DRR028351_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028351_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1958217
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57830.2953196709047057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC24800.053.4646454
ATCAACG25000.051.878983
GTATCAA27250.050.0051231
CAACGCA27450.048.8336225
GGTATCA16800.047.4576871
AACGCAG28500.046.0187076
TATCAAC34300.039.5117382
ACGCAGA32300.038.3613857
CGCAGAG33300.035.9037978
GTACATG46050.034.7845041
TACATGG46400.032.0194972
GCAGAGT39900.025.9735099
CATGGGG42800.024.7158954
CGTTATA2351.3357858E-624.6717932
ACATGGG56450.024.6442573
GAGTACA35550.021.4078431
GTATTAG14050.020.6351131
GTATAAG11150.019.5015681
ATGGGCG3703.4207424E-617.625055
TAACGGA2901.8754357E-417.4890944
Produced by FastQC (version 0.11.5)