FastQCFastQC Report
Thu 2 Jun 2016
DRR028351_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028351_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1958217
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46350.23669491174880003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40050.20452278782177868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21110.10780214858720968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29350.089.710021
GTATCAA61900.065.972091
TCAACGC72700.055.924854
ATCAACG73450.055.1564643
CAACGCA75600.053.034345
AACGCAG76800.052.3000836
TATCAAC78900.051.162832
ACGCAGA87100.045.948867
CGCAGAG88250.045.432258
GCAGAGT102550.038.036499
GTACATG45750.035.196891
TACATGG46450.031.8288422
ACATGGG47800.030.929913
CATGGGG42750.026.2767684
AACGCCG1751.2560151E-424.858056
CAGAGTA100050.024.3487159
GTGGTAT15200.023.8599151
TGGTATC14400.023.1511042
CGTACAC2455.131903E-520.7065773
ATGGGGG33000.020.652275
Produced by FastQC (version 0.11.5)