FastQCFastQC Report
Thu 2 Jun 2016
DRR028350_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028350_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778679
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50760.2853803300089561No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17200.050.1328164
ATCAACG17850.048.7131923
CAACGCA18850.046.899085
GTATCAA21500.045.8460661
AACGCAG20800.041.1087726
GGTATCA14650.037.599111
GTACATG37450.037.544951
ACGCAGA23200.035.919127
TATCAAC26550.034.9389842
CGCAGAG23350.034.7583548
TACATGG40550.030.7398992
ACATGGG44000.027.0081443
CATGGGG35900.025.2301084
TCTGTCG5950.023.1400728
TACCGTC1650.002609383521.9584627
ATTACGC1650.002609811321.957843
GCAGAGT34950.021.3571179
GAGTACA28950.019.026841
GTATAAG10300.018.2952251
CTGTCGC6900.017.8546949
Produced by FastQC (version 0.11.5)