FastQCFastQC Report
Thu 2 Jun 2016
DRR028350_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028350_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778679
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35200.197899677232373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30980.17417420456417373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.077.5119861
GTATCAA47900.059.809991
TCAACGC53850.052.4896284
ATCAACG54500.051.996593
CAACGCA55550.050.2436455
TATCAAC59700.047.7103732
AACGCAG58750.047.5069736
ACGCAGA66000.041.8490457
CGCAGAG66950.040.8220948
GCAGAGT78050.034.4592259
GTACATG36900.031.2523651
TACATGG36950.028.6373622
ACATGGG39900.026.3384173
GTGGTAT13750.025.8467241
CAGAGTA76900.023.473489
CATGGGG34800.022.9091014
TGGTATC14600.021.8421022
GAGTACA28250.020.7959461
AACGCCG1900.00589227719.0774756
AGAGTAC71400.018.77007910-14
Produced by FastQC (version 0.11.5)