FastQCFastQC Report
Thu 2 Jun 2016
DRR028349_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028349_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908701
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57160.29947068713224334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17550.047.895484
ATCAACG18000.046.699323
CAACGCA18950.044.3581965
AACGCAG20750.040.5113226
GGTATCA15700.039.2426761
GTATCAA23850.037.6853561
GTACATG39400.036.0577961
ACGCAGA24350.033.6309287
CGCAGAG24000.033.5201038
TATCAAC26900.032.063522
TACATGG42450.029.538262
TAGACCG1658.45062E-526.3507165
ACATGGG47350.025.4046483
GTATTAG13950.024.4209751
CATGGGG39900.023.0645244
CCTATAC8100.022.3655763
GCAGAGT37950.019.6717019
TCTGTCG7000.018.6366628
TATACGG2050.00914406817.677982
ATGGGGG30450.016.8964755
Produced by FastQC (version 0.11.5)