FastQCFastQC Report
Thu 2 Jun 2016
DRR028349_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028349_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908701
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34960.18316121802209986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30290.15869431618676785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26350.072.1115951
GTATCAA48750.058.4658621
TCAACGC54950.051.171694
ATCAACG55300.051.1099243
CAACGCA56050.050.047315
AACGCAG58850.048.1588026
TATCAAC63100.044.7920572
ACGCAGA65900.042.7867777
CGCAGAG66550.042.3688778
GTACATG38900.035.0503771
GCAGAGT79650.034.7634629
TACATGG41250.030.9210682
ACATGGG42250.030.1892093
GTGGTAT16200.027.756271
CATGGGG37950.027.6899384
TGGTATC15400.026.353182
CAGAGTA76650.024.3033629
GACCGCG2556.973138E-519.8977187
GGAGCGT2959.587324E-619.6568268
CTAGTAC5900.019.6532213
Produced by FastQC (version 0.11.5)