FastQCFastQC Report
Thu 2 Jun 2016
DRR028348_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028348_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908550
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57240.29991354693353595No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19850.050.007814
ATCAACG21250.046.031233
CAACGCA22100.044.263135
GTATCAA24900.043.0846861
GGTATCA15000.042.0425071
AACGCAG23450.041.098016
GTACATG40100.038.141581
TATCAAC29900.034.6567882
ACGCAGA26150.034.640547
CGCAGAG26300.033.616348
TACATGG42700.033.2622532
CATGGGG42200.025.4113084
ACATGGG49700.025.22133
GCAGAGT36100.022.886449
TATACCG2205.927835E-419.761925
GTATTAG15050.019.2656691
ATGGGGG35100.019.1989065
TAGTACG4553.4651748E-919.1094264
GTATAGA14650.017.8125231
ATTAGAC11100.017.624573
Produced by FastQC (version 0.11.5)