FastQCFastQC Report
Thu 2 Jun 2016
DRR028348_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028348_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908550
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44250.2318514055172775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34620.18139425218097507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20680.10835450996830054No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.079.319791
GTATCAA56600.066.755161
ATCAACG63900.058.414353
TCAACGC63650.058.302174
CAACGCA66950.054.907275
AACGCAG69350.053.0070886
TATCAAC72550.051.449712
ACGCAGA76450.047.7048957
CGCAGAG76700.047.5494048
GCAGAGT91150.039.6136789
GTACATG39900.033.0797351
TACATGG43650.029.3901752
ACATGGG44000.027.1796843
CATGGGG36600.025.1498724
CAGAGTA88650.023.9641119
CTATACG2806.079821E-620.7202721
AGAGTAC82900.019.58312410-14
GAGTACT75700.018.29983510-14
CTGTACG2450.001216827517.756519
GTGGTAT16550.017.5277231
Produced by FastQC (version 0.11.5)