FastQCFastQC Report
Thu 2 Jun 2016
DRR028347_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028347_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572769
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47280.3006163015674902No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18300.051.863984
ATCAACG18500.051.304923
GTATCAA20900.050.2924771
CAACGCA20600.046.7812235
AACGCAG20900.046.1097266
GTACATG40300.039.393121
ACGCAGA23550.039.386547
CGCAGAG23250.038.960968
TATCAAC27000.038.3845522
GGTATCA12850.037.796621
TACATGG42350.035.253162
ACATGGG44450.030.9700053
GCAGAGT32300.026.0262749
CATGGGG35150.024.7344074
GAGTACA28150.022.6613371
TATACCG1600.002183011322.643175
CATGGGA19650.020.2789614
TACACGG3953.144487E-718.3438325
GTATTAG13400.017.8521461
CCGGTCG3352.8701766E-517.3061989
Produced by FastQC (version 0.11.5)