FastQCFastQC Report
Thu 2 Jun 2016
DRR028347_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028347_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572769
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48930.3111073527008734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37540.23868730881648864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22910.14566665543382404No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.085.699641
GTATCAA49950.070.566541
ATCAACG60400.057.8368033
TCAACGC61400.056.658764
CAACGCA63600.054.4952135
TATCAAC66550.052.6009142
AACGCAG66750.052.2494056
CGCAGAG71600.048.1025548
ACGCAGA72250.047.8705147
GCAGAGT85450.039.3725439
GTACATG39050.034.1739231
TACATGG39600.032.3945732
ACATGGG38450.032.0440063
TACGGGC4200.027.6221988
ACGGGCC4450.026.0703899
ATACGGG4450.026.0703897
CATGGGG31250.025.7434754
CAGAGTA84550.025.0412949
AATACGG4850.023.9202546
ACCGAGT1550.001807747323.3897658
Produced by FastQC (version 0.11.5)