FastQCFastQC Report
Thu 2 Jun 2016
DRR028346_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028346_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12124
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2932.416694160343121No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA550.45364566149785546No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA480.395908940943583No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA410.33817222038931044No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN350.28868360277136257No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA350.28868360277136257No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC260.2144506763444408No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.2144506763444408No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA250.20620257340811612No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.17321016166281755No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG210.17321016166281755No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA210.17321016166281755No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC200.16496205872649292No Hit
GTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAAC200.16496205872649292No Hit
GTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATA190.15671395579016828No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA180.14846585285384362No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG170.14021774991751898No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC170.14021774991751898No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT170.14021774991751898No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA160.13196964698119432No Hit
GCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTG160.13196964698119432No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT160.13196964698119432No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG150.1237215440448697No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.1237215440448697No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC140.11547344110854503No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT140.11547344110854503No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG130.1072253381722204No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG130.1072253381722204No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT130.1072253381722204No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACA100.006989992145.14
ACACCAC100.006989992145.18
GGACAAT100.006989992145.16
GACACCA100.006989992145.17
GAATCAA100.006989992145.11
AGACACC100.006989992145.16
GTACAAG151.1968839E-4145.099991
GTATCAA256.1849278E-6116.080011
ATCAACA259.135697E-487.0600053
ACAACAC259.135697E-487.0600053
TATCAAC406.3769694E-572.552
TCAACAC300.001884147172.5499954
TAACAAC451.142714E-464.488891
ACGCAGA400.005890705654.4125027
TCAACGC400.005890705654.4125024
GCAGAGT400.005890705654.4125029
CGCAGAG400.005890705654.4125028
TTAGGTG400.005890705654.4125025
ATCAACG400.005890705654.4125023
GGTGAGA553.0818442E-452.7636348
Produced by FastQC (version 0.11.5)