FastQCFastQC Report
Thu 2 Jun 2016
DRR028345_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028345_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431570
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42970.3001599642350706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG14500.056.46313
TCAACGC14850.055.622174
CAACGCA16400.049.9233975
AACGCAG17600.045.6977776
GTATCAA19950.044.327081
GGTATCA11650.042.3092351
ACGCAGA19750.038.5245367
CGCAGAG19550.037.0679748
TATCAAC22250.036.1575932
GTACATG30000.033.826651
TACATGG31950.028.8097442
GCAGAGT28800.023.9051789
CATGGGG30800.023.5244164
ACATGGG38300.022.8897443
GAGTACA23150.019.0998971
GTCTAAG5601.2551027E-1018.121421
GTATTAG11700.017.3471
TAATACT19400.017.1800984
TATACTG14500.016.9894925
GAGTGCG3002.4272675E-416.907997
Produced by FastQC (version 0.11.5)