FastQCFastQC Report
Thu 2 Jun 2016
DRR028345_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028345_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431570
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26980.18846441319670013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22650.1582179006265848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20300.082.878771
GTATCAA37450.062.352721
ATCAACG44300.052.8417323
TCAACGC44400.052.559494
CAACGCA46250.049.5411385
AACGCAG47000.048.5963176
TATCAAC50600.046.1193962
ACGCAGA52700.043.064997
CGCAGAG53750.042.088828
GCAGAGT64350.034.4797029
GTACATG30450.034.2946621
TATACCG850.002740749934.121785
TACATGG33300.029.598722
ACATGGG33650.029.0754833
TCTAGCG1306.498289E-427.874362
CATGGGG29800.026.5087054
GTGGTAT10600.023.9449271
TGGTATC10800.023.4867272
TATACGG1600.002180261522.6479172
CAGAGTA61900.022.607759
Produced by FastQC (version 0.11.5)