FastQCFastQC Report
Thu 2 Jun 2016
DRR028343_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028343_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039695
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60490.29656394706071254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16800.052.1867564
ATCAACG17450.050.6580663
CAACGCA19200.045.664535
AACGCAG20600.042.561126
GTATCAA22700.041.8301961
GTACATG43000.039.7821271
ACGCAGA23100.037.0148167
TACATGG44900.035.0271222
TATCAAC27650.033.2889142
CGCAGAG24200.032.937728
GGTATCA15900.032.367241
ACATGGG52450.028.0436613
CATGGGG44700.024.8009384
TATACCG1952.625959E-422.2952085
CTAGTAC6250.022.0271243
GCAGAGT35100.021.0591759
ATGGGGG36050.019.6977085
GTATTAA25950.017.876691
GTACTAG6705.456968E-1217.3097041
GTATAGA15950.017.2690091
Produced by FastQC (version 0.11.5)