FastQCFastQC Report
Thu 2 Jun 2016
DRR028343_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028343_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039695
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34600.16963320496446774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30900.1514932379595969No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25850.077.4345551
GTATCAA49150.064.187731
ATCAACG55950.055.3101923
TCAACGC56450.054.820294
CAACGCA59400.051.3870935
AACGCAG60350.050.5781866
TATCAAC63850.048.807322
ACGCAGA67500.044.7909977
CGCAGAG67800.044.6997458
GCAGAGT83050.035.6187749
GTACATG41500.034.252651
TACATGG46050.029.5873182
GTCGTAC1907.141245E-626.7196461
ACATGGG47450.026.576053
CAGAGTA82500.022.4980289
CTAGTAC5950.021.9246643
CATGGGG42950.020.7548374
TCGTACA3208.5097236E-720.3830852
GTACCGT1850.005047268719.5955166
GTATTAG16150.018.8609261
Produced by FastQC (version 0.11.5)