FastQCFastQC Report
Thu 2 Jun 2016
DRR028342_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028342_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559641
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45490.29166968552378403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC15350.049.559514
ATCAACG16600.045.3926283
AACGCAG18100.042.4354976
CAACGCA17950.042.38375
GGTATCA12750.042.062341
GTACATG39800.040.9705051
GTATCAA21000.040.722521
TACATGG41450.036.0141032
TATCAAC23300.035.4551122
ACGCAGA22100.033.1154867
ACATGGG44300.033.037543
CGCAGAG21950.033.0137948
CATGGGG37800.028.5588424
GCAGAGT31800.020.9654719
ATGGGGG34600.020.9410255
GAGTACA27700.020.1457211
GTATAGA13600.019.716721
CGTAGAC2256.8996754E-419.3209653
TCACCGG3252.213345E-517.837606145
GTATTAG12700.017.6900831
Produced by FastQC (version 0.11.5)