FastQCFastQC Report
Thu 2 Jun 2016
DRR028342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559641
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38200.24492815974958337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32360.20748364527477797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18050.11573176134764347No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24900.076.610131
GTATCAA43350.058.561031
ATCAACG49500.051.099393
TCAACGC50300.050.574854
CAACGCA51950.047.864715
AACGCAG53450.046.3858266
TATCAAC55550.045.403642
ACGCAGA61500.040.1963047
CGCAGAG62200.039.8604858
GTACATG36650.038.591391
TACATGG37150.036.872152
GCAGAGT72400.033.3436059
ACATGGG41800.031.7300153
GTACGAC1255.1650585E-428.9904843
CATGGGG35050.028.1220114
GTGGTAT13100.023.808221
TGGTATC12350.023.4740772
CAGAGTA71500.022.6102689
AACGCCG2003.1056185E-421.7484426
ACGCCGA2053.6694857E-421.217997
Produced by FastQC (version 0.11.5)