FastQCFastQC Report
Thu 2 Jun 2016
DRR028341_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028341_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550632
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45630.2942671117325065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12650.050.99421
TCAACGC19450.050.2980464
GTATCAA21650.049.882671
ATCAACG19800.049.408943
AACGCAG22050.044.3657766
CAACGCA22700.044.054495
TATCAAC26000.040.6979642
GTACATG41100.038.4446561
ACGCAGA26250.036.716337
CGCAGAG26150.036.3036778
TACATGG39350.036.0997772
GTCGTAC1352.146893E-532.2135661
TATAACG1000.006085371628.9903322
CATGGGG37000.025.8528964
GCAGAGT33700.025.16069
ACATGGG51250.023.7548543
TCGTACA2552.7165242E-622.7375142
CGTACAC2003.113825E-421.7399333
GAGTACA30650.020.1006361
GTATACG3001.108729E-519.3281381
Produced by FastQC (version 0.11.5)