FastQCFastQC Report
Thu 2 Jun 2016
DRR028341_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028341_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550632
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41270.2661495441858545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36020.23229238142899153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18390.11859680439975442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24950.091.273961
GTATCAA51650.065.57441
ATCAACG61850.054.137213
TCAACGC61950.053.932834
CAACGCA64750.051.1726725
AACGCAG66700.050.0027246
TATCAAC67350.049.6085932
ACGCAGA75350.043.973867
CGCAGAG75550.043.7614828
GCAGAGT88400.036.908129
TGGTATC12200.029.109172
GTACATG42050.028.630561
AACGCCG1050.007707689427.6205546
GTGGTAT14050.027.3581891
TACATGG41200.026.738682
CAGAGTA86800.025.1424989
ACATGGG44050.024.0215233
CATGGGG36550.023.7950954
GAGTACA32300.019.9836711
AGAGTAC82350.018.85097710-14
Produced by FastQC (version 0.11.5)