FastQCFastQC Report
Thu 2 Jun 2016
DRR028340_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028340_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686000
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50830.30148279952550416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.050.655141
ATCAACG18350.047.77913
TCAACGC18850.046.510364
AACGCAG21100.042.5834476
CAACGCA21150.041.453735
GTATCAA23600.040.5396421
GTACATG37450.036.1915971
CGCAGAG23450.034.609058
TACATGG39250.033.7881742
ACGCAGA25400.033.6637737
TATCAAC28350.032.4641462
ACCGCGT1255.1708653E-428.9850798
ACATGGG42950.027.3300553
CATGGGG32400.025.7174874
GCAGAGT31800.022.7883689
ATACCGT2303.1569005E-522.0532076
GTATATA19250.021.085081
TCGCCGG4402.2828317E-919.76138145
GAGTACA29000.019.244681
ACGGGCC4553.4578989E-919.1121799
Produced by FastQC (version 0.11.5)