FastQCFastQC Report
Thu 2 Jun 2016
DRR028340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686000
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42430.25166073546856466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35120.20830367734282323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19500.11565836298932383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.088.3188551
GTATCAA50850.062.052121
ATCAACG59350.052.875183
TCAACGC59800.052.7196734
CAACGCA62700.049.839625
AACGCAG65050.048.262036
TATCAAC68200.045.6950072
CGCAGAG71400.044.0713658
ACGCAGA71950.043.532947
GCAGAGT84450.036.4883549
GTACATG36600.035.2774541
ACATGGG35950.032.658843
TACATGG38500.031.2487052
TGGTATC10000.028.9897652
CATGGGG31800.026.8930194
GTCGTAC1100.00964953726.3770161
GTGGTAT12950.025.2058351
CAGAGTA84350.024.1538439
GTATATA18100.021.2400551
GGACGGT2053.6663393E-421.2208356
Produced by FastQC (version 0.11.5)