FastQCFastQC Report
Thu 2 Jun 2016
DRR028339_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028339_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659323
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48950.29499982824320525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.044.3461841
TCAACGC17400.042.055754
ATCAACG17800.041.1106763
CAACGCA19950.037.0456125
GTATCAA21850.035.8345151
AACGCAG20500.034.9903076
GTACATG37450.033.103471
ACGCAGA22850.030.7603557
TATCAAC25450.030.190372
CGCAGAG23500.029.2928478
TACATGG38900.028.3233342
ACATGGG43700.023.5429133
CGATTAC1650.002601876421.9692521
CATGGGG37800.020.8923844
GCAGAGT31800.020.5116219
GAGTACA27850.019.2635231
GTATTAG12000.017.520481
TCTGTCG5902.6739144E-1017.1941958
TACGGGT3052.7568982E-416.6284394
ATGGGGG32500.016.4978715
Produced by FastQC (version 0.11.5)