FastQCFastQC Report
Thu 2 Jun 2016
DRR028339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659323
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41150.2479927054587925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34450.20761479229782268No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17830.10745346144180488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26700.078.775861
GTATCAA49550.062.501531
ATCAACG56400.054.483613
TCAACGC56500.054.387184
CAACGCA58100.052.1582835
AACGCAG59600.051.0888566
TATCAAC63850.048.3534932
ACGCAGA68200.044.4339567
CGCAGAG68650.044.1426938
GCAGAGT79550.037.274029
GTGGTAT14000.026.9390161
GTACATG34750.026.9241091
TGGTATC13750.026.3539942
TACATGG35550.026.094532
ACATGGG35950.024.5946183
CAGAGTA77200.022.8198159
GTACGAC1650.002607106721.9616623
TTACCCG1700.003094259421.3227985
CATGGGG31750.020.5436634
GAGTACA27300.019.9253081
Produced by FastQC (version 0.11.5)