FastQCFastQC Report
Thu 2 Jun 2016
DRR028338_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028338_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580328
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46550.2945591041859665No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16250.056.6220554
ATCAACG16950.055.138543
AACGCAG18900.049.8361366
CAACGCA19300.048.426325
ACGCAGA20550.044.4286277
GTATCAA22650.044.1610371
CGCAGAG21550.042.030738
TATCAAC25400.037.378732
GGTATCA13300.033.7884641
GTACATG32550.033.1790281
TACATGG33250.030.5157032
GCAGAGT28750.029.2382899
GAGTACA26700.023.6176321
ACATGGG42700.021.8875483
TATACGG2353.7300146E-521.581385
TACTCCG3106.2887557E-721.0343865
CCTATAC7250.020.9853653
CATGGGG34950.020.7294644
TATACTG14200.020.408955
AGTACAT30600.018.9476592
Produced by FastQC (version 0.11.5)