FastQCFastQC Report
Thu 2 Jun 2016
DRR028338_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028338_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580328
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37270.23583711735791554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31420.19881948557514645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17520.11086306133916504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.081.2770541
GTATCAA46350.064.161771
ATCAACG53250.055.529713
TCAACGC53850.054.7764054
CAACGCA55700.052.4515575
AACGCAG56850.051.645576
TATCAAC61050.048.5537152
ACGCAGA65700.044.6887477
CGCAGAG67050.043.572738
GCAGAGT75400.037.6897479
GTACATG32600.032.0396041
GTACGAC1652.394163E-630.7467293
TACATGG34500.029.1998442
ACATGGG37050.026.407693
CATGGGG31700.026.2919864
TGGTATC11450.024.6856172
CAGAGTA74300.024.5878339
GTGGTAT14400.022.1631971
TACGACC2653.793799E-621.8790764
CGACCAT3055.3477015E-721.391996
Produced by FastQC (version 0.11.5)