FastQCFastQC Report
Thu 2 Jun 2016
DRR028337_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028337_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581400
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45110.28525357278360947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14950.059.124584
ATCAACG15250.058.438423
GTATCAA18800.053.2031
CAACGCA17150.052.393325
AACGCAG18700.048.0490386
ACGCAGA21350.041.0695237
CGCAGAG21100.040.528388
GGTATCA11800.038.082421
TATCAAC25050.037.61172
GTACATG35450.037.210891
TACATGG37300.030.8941042
CATGGGG34450.027.1300094
GCAGAGT29100.025.1553029
ACATGGG43050.024.9087433
ATGGGGG27600.019.9536745
GAGTACA25950.019.8306031
GTCTCGC3852.3943176E-718.8258321
GCTATAC5056.2573235E-1018.6515623
TAACCGA1950.006863517718.5797546
CGCTCTG5601.2732926E-1018.1158375
Produced by FastQC (version 0.11.5)