FastQCFastQC Report
Thu 2 Jun 2016
DRR028337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581400
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40410.25553307196155306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35020.22144934867838625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19410.12273934488427976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26800.086.342551
GTATCAA49650.061.5080381
ATCAACG58100.052.392763
TCAACGC59100.051.751524
CAACGCA60850.049.923225
AACGCAG63350.048.296436
TATCAAC64900.046.903222
ACGCAGA69900.043.459637
CGCAGAG70500.043.398288
GCAGAGT82150.036.626029
GCGTTAC900.003628406732.2230536
GTACATG35650.027.4690251
GTGGTAT12550.027.165841
CGACACG1358.0835854E-426.8525439
TGGTATC11700.025.39782
TACATGG37700.024.7997022
CAGAGTA79150.024.7321599
ACATGGG37350.024.6440033
TCTGTCG4403.6379788E-1223.0687778
CATGGGG31500.021.627954
Produced by FastQC (version 0.11.5)