FastQCFastQC Report
Thu 2 Jun 2016
DRR028336_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028336_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807779
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53360.29516882318026705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22750.052.2393234
ATCAACG23850.049.8299643
GTATCAA25150.049.2766841
GGTATCA15150.048.3161241
CAACGCA25300.046.691565
AACGCAG26200.045.0889056
TATCAAC30600.039.5518072
ACGCAGA30250.038.814787
GTACATG48550.035.0801961
CGCAGAG32000.034.653658
TACATGG50400.032.2098542
ACATGGG54950.027.4303113
CATGGGG44750.024.4522614
GAGTACA36200.024.4250031
GCAGAGT40300.024.1001269
CTAACGT1750.003669771820.704614
AGTACAT41150.020.6056962
GTATATA17600.019.7656881
ATATCGC1850.00505882119.5876186
GTACGAC1850.005062072519.5854423
Produced by FastQC (version 0.11.5)