FastQCFastQC Report
Thu 2 Jun 2016
DRR028336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807779
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45520.25180069023923835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37790.20904103875529034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21530.11909641609953431No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28500.083.2247241
GTATCAA54250.063.7775881
TCAACGC65000.053.0770264
ATCAACG65700.052.40123
CAACGCA68250.050.1400155
TATCAAC69150.049.5771942
AACGCAG70150.048.885336
ACGCAGA77700.043.761987
CGCAGAG80650.042.4309548
GCAGAGT92900.036.4456989
GTACATG50100.035.90571
TACATGG50950.034.7103232
ACATGGG53400.032.3034323
CATGGGG44350.029.743414
CAGAGTA90700.024.77999
GTGGTAT13700.020.6487371
TAAGCGA1950.00684199418.5900485
AGTACCG1950.00684199418.5900485
TATAACG1950.006854019618.5843942
AGAGTAC86800.018.49955210-14
Produced by FastQC (version 0.11.5)