FastQCFastQC Report
Thu 2 Jun 2016
DRR028335_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028335_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488475
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43420.2917079561295957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11400.050.225371
TCAACGC18250.048.839354
ATCAACG18950.047.798453
GTATCAA21350.044.8102071
AACGCAG20600.042.5643276
CAACGCA21800.040.553755
GTACATG42600.039.130841
TATCAAC25750.037.1520652
ACGCAGA23900.036.3840457
TACATGG44150.035.6217542
CGCAGAG25050.034.713728
ACCGCGT1301.6608137E-533.445338
ACATGGG49150.030.8131263
CATGGGG42800.029.6293374
ATGGGGG32700.024.1549635
GCAGAGT33000.023.4977769
CCGTTAC1800.004315913720.1325321
GTATATA11950.020.01461
CAGAGTA31000.019.6369749
GAGTACA31200.019.0484711
Produced by FastQC (version 0.11.5)