FastQCFastQC Report
Thu 2 Jun 2016
DRR028335_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028335_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488475
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50140.3368548346461983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40370.27121718537429246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25200.1693007944372596No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25700.087.775431
GTATCAA52150.070.7960741
ATCAACG62250.058.2138143
TCAACGC62400.058.1900254
CAACGCA65250.055.4504555
TATCAAC66550.054.6702462
AACGCAG67100.054.2458236
ACGCAGA75050.048.4029857
CGCAGAG77700.046.565548
GCAGAGT85750.040.672059
GTACATG45550.034.555491
TACATGG45700.033.1455732
ACATGGG48200.030.9753043
CATGGGG40850.028.9195124
CGTACCC1100.00968856426.3549823
CAGAGTA84000.024.6008829
TCTATAC10850.023.379423
GAGTACA32350.022.6460671
TATACGT1650.002599812821.972085
GTACGAC1700.003099391721.316533
Produced by FastQC (version 0.11.5)