FastQCFastQC Report
Thu 2 Jun 2016
DRR028334_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028334_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334833
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38910.2914971385933671No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13600.049.017234
ATCAACG13700.048.659443
CAACGCA15400.044.228985
AACGCAG15850.042.057366
GTATCAA17400.040.4051481
GTACATG30950.036.064031
TATCAAC19500.035.307792
TTAGCGC1051.8587944E-434.5087748
TACATGG31550.033.9972572
ACGCAGA18500.033.686767
CGCAGAG18750.032.0793578
GGTATCA12900.031.4638981
GTACTAG3153.45608E-1127.6111771
ACATGGG38250.026.9002763
CATGGGG30900.025.3259124
CTAGTAC4103.8198777E-1122.97523
GCAGAGT25600.022.0810579
GAGTACA22950.019.896291
ATAATAC12950.019.583853
GTATAAT12300.018.8564131
Produced by FastQC (version 0.11.5)