FastQCFastQC Report
Thu 2 Jun 2016
DRR028334_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028334_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334833
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27210.20384572452134464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22930.17178178843345945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18150.081.1053541
GTATCAA34400.060.710561
TCAACGC40350.050.832944
ATCAACG41100.050.0816763
CAACGCA41800.048.9108665
AACGCAG43700.047.4479146
TATCAAC45950.044.953312
ACGCAGA50250.040.686057
CGCAGAG50400.040.133428
GTACATG31550.034.93611
GCAGAGT60900.032.6186339
TACATGG32800.032.261262
ACATGGG34250.030.260623
CAGAGTA58500.024.1663599
CATGGGG30700.024.0804254
GAGTACA26100.022.2269381
GTGGTAT9400.020.0574471
TATACAG13550.019.7967975
TCTATAC8700.019.1606793
AGTACAT27750.019.0661142
Produced by FastQC (version 0.11.5)