FastQCFastQC Report
Thu 2 Jun 2016
DRR028333_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028333_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391572
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41740.29994854739819427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17150.052.391214
GGTATCA12300.050.6816671
ATCAACG18000.049.917183
AACGCAG18450.047.5197536
CAACGCA19850.045.6316345
GTATCAA21050.045.4547651
TACATGG35450.042.3187982
GTACATG37350.041.53181
ACGCAGA22050.038.122527
TATCAAC23650.037.998792
CGCAGAG21600.036.5683148
ACATGGG40150.035.7339063
CATGGGG36500.031.3664284
ATGGGGG29500.025.0550165
GCAGAGT28900.023.8260489
GTATTAG8450.020.58791
CTATACT10600.020.5076684
ATTGCGT2908.271636E-619.9948529
GAGTACA22250.019.8727251
CCTATAC5759.094947E-1218.9027183
Produced by FastQC (version 0.11.5)