FastQCFastQC Report
Thu 2 Jun 2016
DRR028333_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028333_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391572
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32590.2341955716269083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26350.1893541979861624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14980.1076480412080726No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19850.083.3112951
GTATCAA40450.064.5525741
ATCAACG44650.057.4603043
TCAACGC45550.056.6431924
AACGCAG47850.052.727786
TATCAAC49300.052.3346372
CAACGCA48350.052.3324625
ACGCAGA56650.044.2810977
CGCAGAG57800.043.4000748
GTACATG38700.037.484231
GCAGAGT66400.036.5779159
TACATGG39350.034.625752
ACATGGG39900.033.4218943
CATGGGG35750.030.4089434
GTGGTAT9700.027.666841
GAGTACA25200.025.6164551
CGATACA1751.2590569E-424.8484524
TGGTATC10700.024.3839952
TATACCG1500.001494482324.1668995
ATGGGGG29600.022.7789365
Produced by FastQC (version 0.11.5)