FastQCFastQC Report
Thu 2 Jun 2016
DRR028332_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028332_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413886
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42530.3008021863148797No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC12750.055.7008484
ATCAACG13500.053.681863
CAACGCA14350.050.0006335
GTATCAA17650.046.8114781
AACGCAG15500.044.8865556
GTACATG30800.044.47371
TATCAAC20300.039.2711332
ACGCAGA18200.039.0253567
CGCAGAG17800.037.86658
GGTATCA11100.037.217231
TACATGG33650.036.828822
TACGCAG1902.0837251E-730.516075
ACATGGG43200.026.8409333
CATGGGG34450.026.0842514
GCAGAGT26650.022.028259
CGAGCGG3355.520269E-821.635294145
ATAGTAC8300.020.955333
TCTATAC9000.019.3254723
GTATTAG10250.019.091151
GAGTACA23600.018.7331161
Produced by FastQC (version 0.11.5)