FastQCFastQC Report
Thu 2 Jun 2016
DRR028332_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028332_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413886
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25030.17702983125938018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23040.1629551463130691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20200.074.317491
GTATCAA38100.062.4339681
TCAACGC42850.054.633954
ATCAACG43150.054.2541053
CAACGCA44100.052.769735
AACGCAG46600.050.0943036
TATCAAC47500.049.2855722
ACGCAGA52600.044.3801277
CGCAGAG53450.043.5387278
GCAGAGT62250.035.869879
GTACATG30500.032.5756261
TACATGG31300.029.6398852
ACATGGG35100.026.0180263
GTGGTAT10450.023.5957981
CATGGGG32950.023.0964244
TGGTATC10700.022.353272
CAGAGTA62450.021.7083859
TCCGTTC1800.004309063320.137988
TACATCG2608.083131E-519.5183495
CCTATAC5605.456968E-1219.413963
Produced by FastQC (version 0.11.5)