FastQCFastQC Report
Thu 2 Jun 2016
DRR028331_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028331_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403937
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40620.289329222037741No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC15200.047.670944
ATCAACG16050.045.146313
GGTATCA11900.045.0783961
AACGCAG17000.043.0527426
GTATCAA19200.042.286381
CAACGCA18150.040.3234445
ACGCAGA18450.039.279247
CGCAGAG19750.035.9598968
TATCAAC21850.035.4926452
GTACATG35650.035.177931
TACATGG33700.033.980772
ACATGGG35800.029.9554633
CATGGGG32600.026.672334
ATTACGC1100.00969903926.3490313
GCAGAGT25600.024.3454579
GTATAGA8950.024.2986371
TACCGTA2101.5606049E-524.1567337
TAGCGCA1550.00181468923.374144
GTATTAG11700.020.4461611
GTCTCGC3301.1400098E-619.7702541
Produced by FastQC (version 0.11.5)