FastQCFastQC Report
Thu 2 Jun 2016
DRR028331_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028331_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403937
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39120.27864498193295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33370.23768872819791773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20120.1433112739389303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.081.030991
GTATCAA44050.062.098591
TCAACGC51750.051.820064
ATCAACG52050.051.799883
CAACGCA54500.048.816765
AACGCAG55350.048.4600076
TATCAAC56250.047.9321562
ACGCAGA61200.043.4724437
CGCAGAG62400.041.9393778
GCAGAGT71300.036.195949
GTACATG32700.033.9492381
TACATGG32400.033.330962
ACATGGG35550.030.1737083
CATGGGG31900.024.765234
CAGAGTA71500.024.637679
GTGGTAT11650.021.7985921
TGGTATC11900.020.7080172
GTATATA13150.019.8638341
GTATTAG10550.019.2571451
GAGTACA24900.019.2322861
Produced by FastQC (version 0.11.5)