FastQCFastQC Report
Thu 2 Jun 2016
DRR028329_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028329_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18531
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3311.7861961038260215No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN630.3399708596406022No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA480.2590254168690303No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA440.23743996546327772No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA340.18347633694889645No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.16728724839458203No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA270.1457017969888295No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.12951270843451515No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG240.12951270843451515No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG230.124116345583077No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA220.11871998273163886No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT210.11332361988020076No Hit
CTTCTATACAGTACCTTGACTTAAATCCAAGAGCAAAAGTTAAGACTCTC200.10792725702876262No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC200.10792725702876262No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT190.10253089417732449No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG100.007044129144.937845
TATCAAC300.001908988772.468922
CGTATTT300.001908988772.46892145
ATCAACG300.001908988772.468923
GGTGCCC406.5057815E-572.468928
TCAACGC300.001908988772.468924
GTGCCCG406.5057815E-572.468929
TATTGGT350.00351740762.1162154
GTATCAA350.00351740762.1162151
ACGCAGA400.005967919254.351697
TTGGTGC400.005967919254.351696
TGGTGCC400.005967919254.351697
TTATTGG450.00950749848.312613
GTACATG657.1658724E-444.5962561
CTTTAAC200.006059064528.987568115-119
TGCCAAG400.00788277818.1172335-39
GTGGTTT550.002580796115.84566115-19
ATCTCGG1600.004382718.152754125-129
Produced by FastQC (version 0.11.5)