FastQCFastQC Report
Thu 2 Jun 2016
DRR028329_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028329_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18531
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2791.5055852355512385No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2501.3490907128595326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.8256435162700341No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT800.43170902811505046No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.37234903674923103No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.3669526738977929No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA520.28061086827478277No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC500.26981814257190656No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.2590254168690303No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT380.20506178835464897RNA PCR Primer, Index 27 (95% over 23bp)
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA360.19426906265177268No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.16728724839458203No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC280.15109815984026767No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA270.1457017969888295No Hit
GTATAGAAGTTGTGTAAAATCAGTATGAAAGTTCAATGTTGCTGTTCTTG240.12951270843451515No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA230.124116345583077No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.11871998273163886No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.11871998273163886No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA220.11871998273163886No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.11871998273163886No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA210.11332361988020076No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG210.11332361988020076No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT210.11332361988020076No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT200.10792725702876262RNA PCR Primer, Index 27 (96% over 25bp)
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC200.10792725702876262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC200.10792725702876262No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA200.10792725702876262No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG190.10253089417732449No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG190.10253089417732449No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC190.10253089417732449No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA190.10253089417732449No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC190.10253089417732449No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGC100.007058287144.840558
TCTAATT100.007058287144.840553
ATACGGG100.007058287144.840557
GGTATCA650.0111.41581
GTATCAA1350.069.738041
AATCCAG501.9695693E-457.9362145
TATCAAC1750.053.7979162
ATCAACG1850.050.889923
TCAACGC1850.050.889924
CAACGCA2000.047.0731745
AACGCAG2050.045.9250536
TTTCGCG657.189617E-444.5663229
CATTTCG657.189617E-444.5663227
ACATTTC700.001035545641.383016
CGCAGAG2100.041.383018
GACTAAC700.001035545641.383011
ACGCAGA2150.040.420627
TAACATT750.001453870138.6241464
GCAGAGT2300.037.784499
ATTTCGC800.001996227536.2101368
Produced by FastQC (version 0.11.5)