FastQCFastQC Report
Thu 2 Jun 2016
DRR028328_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028328_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317606
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38310.29075459583517377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17800.054.9638064
ATCAACG18600.051.0412643
GGTATCA12650.050.997071
CAACGCA19900.048.0710835
GTATCAA21450.047.985141
AACGCAG20100.046.1523136
GTACATG37900.042.266751
TATCAAC24900.040.4602132
ACGCAGA23200.039.6730237
TACATGG38300.038.794292
CGCAGAG24100.038.1929138
ACATGGG44750.030.6077773
CATGGGG34150.029.497634
GCAGAGT29400.028.1030629
TAGCGCA1902.1985342E-422.8872579
GAGTACA24750.022.8436341
ATGGGGG30350.022.6844335
GTATAGA11550.021.965031
GTATAGG6100.020.2006281
AGTACAT29800.019.700692
Produced by FastQC (version 0.11.5)