FastQCFastQC Report
Thu 2 Jun 2016
DRR028328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317606
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39850.3024424600373708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33910.2573606981146109No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19990.15171454896228462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22800.091.926551
GTATCAA43100.057.884081
ATCAACG50100.049.0429043
TCAACGC50550.048.7497024
CAACGCA52150.047.134725
AACGCAG53550.045.7670336
TATCAAC55000.044.805412
ACGCAGA61250.039.6583147
CGCAGAG62600.038.8030628
GTACATG36850.036.409291
GCAGAGT73100.032.832679
TACATGG38950.032.378452
ACATGGG41150.030.8235343
CATGGGG33350.028.4701464
GTGGTAT10050.024.5352691
TGGTATC9250.024.2903732
CAGAGTA72050.023.8511819
CTAGTAC4851.6370905E-1120.9218713
ACACCGT2857.092638E-620.3535736
GAGTACA27500.020.0428241
Produced by FastQC (version 0.11.5)